槲皮素修饰磁性Fe3O4粒子及其对金属离子检测应用
摘 要
槲皮素是一种广泛存在的黄酮类化合物，由于其具有的多种生物及药理活性而受到广泛关注。研究发现槲皮素具有多种活性，如抗氧化、抗炎、抗肿瘤、肾脏保护。然而，由于槲皮素具有不稳定性、水溶性差，生物利用度低等问题限制了其临床应用。近些年来，纳米给药系统的发展，很大程度上提高了难溶性药物的水中溶解性，提高生物利用度等作用。因此将难溶性药物制备成纳米制剂是提高药物疗效，扩大临床应用非常具有前景的选择。实验主要利用黄酮类化合物，虽然有一定的极性，可溶于水，但却难溶于酸性水，易溶于碱性水，故可用碱性水提取，再将碱水提取液调成酸性，黄酮苷类即可沉淀析出。以槐米为原料，采用煎煮法提取槐米有效成分芦丁，然后酸溶液水解获得槲皮素粗品，乙醇重结晶获得槲皮素精品。文章采用了质谱、高效液相色谱法、红外以及低场核磁对槲皮素进行了表征。由于槲皮素本身具有较低的荧光性能，试验中通过制备了Fe3O4包覆纳米银颗粒，使其包覆槲皮素探究该体系荧光性能是否得到改善。实验配置了不同金属溶液，通过荧光强度以及核磁的测试，观察槲皮素对金属离子的影响。
关键词：槲皮素、纳米银、金属离子、低场核磁、荧光分析
Quercetin modification of magnetic Fe3O4 particles and its application in metal ions detection
ABSTRACT
Quercetin is a flavonoid compounds widely, because it has a variety of biological and pharmacological activity and attracted widespread attention. Study found that quercetin has a variety of activities, such as antioxidant, anti-inflammatory, anti-tumor, kidney care. However, quercetin has no stability, poor solubility in water, low bioavailability problems limited its clinical application. In recent years, the development of nano-drug delivery system, to a large extent, improve the solubility in water insoluble drugs, improve bioavailability, and so on. Poorly soluble drugs into Nano-formulations were prepared to improve drug efficacy and expand the choice of very promising clinical applications. Tests using flavonoid compounds, although there is a certain degree of polarity, which is soluble in water, but insoluble in water, soluble in alkaline water, alkaline water, alkaline solution into acid, flavone glycosides can be precipitated. As raw material to sophora, decocting method to extract rutin from Sophora active ingredients, quercetin and then acid-hydrolysis for crude and ethanol obtained by recrystallization of quercetin products. Using mass spectrometry, HPLC chromatography, infrared and low field NMR of quercetin were characterized. Since quercetin itself has a low fluorescence properties, experiment was prepared by Fe3O4 coated Nano-Silver particles, which covered research on quercetin fluorescence properties of the system improved. Experiments are configured with different metal solution, through fluorescence intensity and the nuclear test and observe the effects of quercetin on the metal ion.
Key words： quercetin, Nano-Silver, metal ions, low-field NMR, fluorescence analysis
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